Mol Microbiol 55(6):1855–1866, Liu G, Chater KF, Chandra G, Niu G, Tan H (2013) Molecular regulation of antibiotic biosynthesis in Streptomyces. 1 Recommendation. Article  2020 Jan 21;10:3123. doi: 10.3389/fmicb.2019.03123. Blue white screening - Foriegn DNA and plasmid Dna are cut with restriction enzyme. Mol Microbiol 25:1177–1184, Yang N (2014) The study of bacteria diversity from the south China sea and Xinjiang desert and chemical investigation of one Streptomyces strain. Blue-white screening is just that  a screening process. Gene 146:105–110, Ralph K, Vartul S, Nicholas PT, Jolanta ZC, Katarzyna W, Paul RH, Chun JC, Govind C, Ahmed HF, Michael G, Paul AH (2012) Draft genome sequence of the human pathogen Streptomyces somaliensis, a significant cause of actinomycetoma. The process of screening bacterial transformants for recombinant plasmids is made more rapid and simple by the use of vectors with visually detectable reporter genes. J Ind Microbiol Biotechnol 40:159–168, Yuriy R, Elke B, Bogdan T, Andriy L (2014) Actinomycetes biosynthetic potential: how to bridge in silico and in vivo? Armistead B, Whidbey C, Iyer LM, Herrero-Foncubierta P, Quach P, Haidour A, Aravind L, Cuerva JM, Jaspan HB, Rajagopal L. Front Microbiol. In order to utilize the α-complementation technique for the purpose of Blue-White screening, you need a few supplies. This work was supported in part by the MOST of China (2013CB734003 and 2015CB150602). Immediate online access to all issues from 2019. Student PhD thesis: University of Chinese Academy of Sciences (China). Get the latest public health information from CDC: https://www.coronavirus.gov. The John Innes Foundation, Norwich, UK, Lambalot RH, Gehring AM, Flugel RS, Zuber P, LaCelle M, Marahiel MA, Reid R, Khosla C, Walsh CT (1996) A new enzyme superfamily—the phosphopantetheinyl transferases. Google Scholar, Marshall VP, Little MS, Johnson LE (1981) A new process and organism for the fermentation production of volonomycin. Blue-White Select ™ Screening Reagent is designed for blue-white selection of recombinant bacterial colonies with the lac+ phenotype. Introduction of foreign DNA into the MCS of phoZMCS produced a white colonial phenotype in E. coli and GBS on agar containing X-p and allowed discrimination between transformants containing recombinant plasmids versus those maintaining self-annealed or uncut vector.  |  PubMed  The plasmid pDC123 containing cpsE was isolated by direct electroporation into GBS strain A909 with selection of transformants containing recombinant plasmids achieved by 'blue/white' screening, without the use of an intermediate host. Background. Mar Biotechnol 11:717–724, Magdevska V, Gaber R, Goranovič D, Kuščer E, Boakes S, Durán Alonso MB, Santamaría RI, Raspor P, Leadlayd PF, Fujs Š, Petković H (2010) Robust reporter system based on chalcone synthase rppA gene from Saccharopolyspora erythraea. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, NO.1 Beichen West Road, Chaoyang District, Beijing, 100101, China, Pengwei Li, Jine Li, Zhengyan Guo, Wei Tang, Jianshan Han, Xiangxi Meng, Tingting Hao, Yaxin Zhu & Yihua Chen, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China, University of Chinese Academy of Sciences, Beijing, 100049, China, Pengwei Li, Wei Tang, Jianshan Han, Xiangxi Meng & Tingting Hao, You can also search for this author in J Biol Chem 282:9073–9081, Thomas JH, Richard HB (1997) Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus. Curr Opin Chem Biol 1:309–315, Wietzorrek A, Bibb M (1997) A novel family of proteins that regulates antibiotic production in streptomycetes appears to contain an OmpR-like DNA-binding fold. Inactivation of the putative γ -butyrolactone synthase gene afsA-y via the idgS -based blue-white screening method revealed that the paulomycin production is negatively controlled by afsA-y in Streptomyces sp. J Bacteriol 184:654–665, Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, Cold spring harbor laboratory press. Article  L.Z. Blue/white screening of recombinant plasmids in Gram-positive bacteria by interruption of alkaline phosphatase gene (phoZ) expression Gene. Combining selection and screening ensures that the white colonies you see are white due to successful cloning and not because the cell failed to take up the α-complementation plasmid. In … Authors D O Chaffin 1 , C E Rubens. Sci China Life Sci 56:619–627, Li J, Xie Z, Wang M, Ai G, Chen Y (2014) Identification and analysis of the paulomycin biosynthetic gene cluster and titer improvement of the paulomycins in Streptomyces paulus NRRL 8115. Below is the link to the electronic supplementary material. 2020 Jul 21;88(8):e00097-20. produced antibacterial substance. YN86. Microb Biotechnol 4:207–215, Hopwood DA (2006) Soil to genomics: the Streptomyces chromosome. Plasmid has gene for lactose hydrolysis lac z gene which codes for enzyme (β-galactosidase). Across the vector’s cloning site lies a DNA sequence encoding a peptide, which can be visually detected as blue colonies. We hypothesized that `blue/white' screening of recombinant plasmids could be accomplished by disruption of the detectable phenotype of the E. faecalis AP gene, phoZ. However, the lack of a suitable chromogenic reporter for Streptomyces has limited the use of color-based screening system to simplify the selection process for double-crossover mutants. Part of Springer Nature. Sarkar P, Danger JL, Jain I, Meadows LA, Beam C, Medicielo J, Burgess C, Musser JM, Sumby P. mSphere. J Ind Microbiol Biotechnol 41:387–402, Zhang H, Zhou W, Zhuang Y, Liang X, Liu T (2013) Draft genome sequence of Streptomyces bottropensis ATCC 25435, a Bottromycin-Producing actinomycete. Chem Biol 3:923–936, Li C, Evans RM (1997) Ligation independent cloning irrespective of restriction site compatibility. PubMed  Li P, Li J, Guo Z, Tang W, han J, meng X, Hao T, Zhu Y, Zhang L, Chen Y (2015) An efficient blue-white screening based gene inactivation system for Streptomyces. In pDC123, phoZMCS was transcriptionally linked to the chloramphenicol acetyl transferase (cat) gene under the control of the constitutively expressed tetM and cat promoters that drive cat expression in pJS3. There are no recommended articles. https://doi.org/10.1007/s00253-014-6369-0, DOI: https://doi.org/10.1007/s00253-014-6369-0, Over 10 million scientific documents at your fingertips, Not logged in J Antibiot 58:1–26, PubMed  doi: 10.1128/AEM.03009-18. Disrupting the LacZ Gene The presence of lactose in the surrounding environment triggers the lacZ operon in E. coli. USA.gov. J Bacteriol 190:6014–6025, CAS  volume 99, pages1923–1933(2015)Cite this article. Inactivation of the putative γ-butyrolactone synthase gene afsA-y via the idgS-based blue-white screening method revealed that the paulomycin production is negatively controlled by afsA-y in Streptomyces sp. doi: 10.1128/mSphere.00519-18. Annu Rev Microbiol 47:685–713, Fang J, Zhang Y, Huang L, Jia X, Zhang Q, Zhang X, Tang G, Liu W (2008) Cloning and characterization of the tetrocarcin a gene cluster from Micromonospora chalcea NRRL 11289 reveals a highly conserved strategy for tetronate biosynthesis in spirotetronate antibiotics. We have developed a Gram-positive cloning vector that utilizes the interruption of an alkaline phosphatase gene, phoZ, to identify recombinant plasmids.

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